Fig 4. The new IL-33 cytokine level was examined from various sources of rat in VILI.

A: in serum, B: in BALF, C: in lung tissue homogenization. The collection samples were determined to IL-33 level by ELISA kit assay. A: Time course of IL-33 in serum in animals exposed to 10 cmH₂O pressure of ventilation. B and C: The expressions of IL-33 in BALF and lung tissue homogenization collected from animals exposed to 10 cmH₂O of ventilation pressure for 4 hours. PC10: animals exposed to VILI via high pressure ventilation at 10 cmH₂O for 4 hours, CTL: control group animals without ventilator administration. *:P <0.05 compared with animals no using ventilator, n = 8. The results are presented in terms of mean±SEM.