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. 2015 Mar 27;10(3):e0122377. doi: 10.1371/journal.pone.0122377

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© 2015 Blázquez et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 2 — The phenotypic analysis was performed by multicolor flow cytometry. The cells were in vitro cultured for 7 days in the presence of Fetal Bovine Serum and in the presence of pericardial fluid at 25%, 50%, 75% and 100%. Representative histograms of four different experiments are shown (A). The expression level of Stem Cell Markers (CD29, CD31, CD44, CD90, CD105) and Swine Leukocyte Antigen Class-I and Class-II (SLA-I and SLA-II) is represented as Normalized Mean Relative Fluorescence Intensity which is calculated by dividing the Mean Fluorescent Intensity (MFI) by its isotype control. Graphic representation of mean±SD for each marker is also provided (n = 4) (B). No significant differences were found between groups when ANOVA test was performed.