Fig 1. A simplified scheme for SLAF-Seq.

(a) Thirty lines with largest TGW and 30 lines with lightest TGW were pooled and used for SLAF-Sequencing (the histogram was drawn based on the data collected at the F₇ generation) (b) All SLAFs (black lines) distributed on 12 chromosomes (c) Polymorphic SLAFs (black lines) distributed on 12 chromosomes (d) Polymorphic markers (black lines) between two DNA bulks distributed on 12 chromosomes with intensive markers distributed in the block of chromosome 3. (e) Identification of the hot region for TGW. The reference genome is in the middle of the map, the green box means intergenic regions, orange box means gene regions, yellow box means repeat regions. Red and blue lines represent the relative abundance of the maternal and paternal alleles between two bulked DNA pools (Pool 1 and Pool 2).