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. 2015 Mar 27;10(3):e0115996. doi: 10.1371/journal.pone.0115996

Fig 4. Inhibition of ApoE and SPARC significantly reduces the growth promoting effects of retinal glial conditioned medium.

Fig 4

(A) Mean length of longest DRG neurite and (B) mean number of DRG after treatment with unconditioned medium, retinal glial conditioned medium or retinal glial conditioned medium which had been pre-incubated with anti-ApoE and/or anti-SPARC antibodies. Photomicrographs of DRG cultured in (C) unconditioned medium, (D) retinal glial conditioned medium, or conditioned medium that had been pre-incubated with (E) anti-ApoE, (F), anti-SPARC or (G) anti-ApoE and anti-SPARC antibodies. Significant differences are indicated by asterisk (P < 0.01 = **; P < 0.001 = ***). Results consist of the means (± SEM) of 3 separate experiments. Adult rat DRG were cultured for 2 days. CM = retinal glial conditioned medium; Scale bars: 100μm.