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. 2015 Mar 27;10(3):e0122209. doi: 10.1371/journal.pone.0122209

Fig 4. Analysis of FOLR1 immunohistochemical staining of breast cancer subtype tissue microarrays.

Fig 4

TMAs comprised of 131 breast cancer tumors of different subtypes from patients with classified breast cancers subtypes that include DCIS (n = 4), ER+ (n = 33), HER2+ (n = 26) and TNBC (n = 68). TMAs were evaluated using high affinity FOLR1 antibody Mab 26B3.F2 and IHC staining intensities calculated as described in the “Materials and Methods”. (A). The percentage of breast tumors with > 30% 3+ staining by subtype. P-values calculated Mann-Whitney test of TNBC versus benign, ER+ and HER2+. (B). H-score distribution of FOLR1 expression. H-scores were calculated with the formula: Hscore = 3 * (% at 3 +) + 2 * (% at 2 +) + 1 * (% at 1 +) + 0 * (% at 0). *Statistical significance determined by Mann-Whitney test of TNBC versus benign, ER+ and HER2+ for both (A) and (B).