Skip to main content
. 2015 Mar 27;10(3):e0122209. doi: 10.1371/journal.pone.0122209

Fig 7. Folate receptor overexpression increases cell growth and folate uptake.

Fig 7

(A). Growth of HS578T breast cancer cells engineered to stably overexpress FOLR1 or empty vector (NT). Cells were cultured in low (0–40 nM) and super-physiological (160 nM) concentrations of folic acid and growth determined after 120 hr using the MTT assay. Error bars represent ± SD. **Statistical significance calculated by two sided unpaired t-test, assuming unequal variances. (B). BrdU incorporation in HS578T/NT and HS578T/FOLR1 cells grown in 10 nM folic acid. **Statistical significance calculated by two sided unpaired t-test, assuming unequal variances. (C). Folate uptake in HS578T/NT versus HS578T/FOLR1 cells. Briefly, flow cytometry was used to measure internalized fluorescent folate (fluorescent tagged folate agent, FolateRSense 690) after 1 hr of exposure. Dot plots show forward scatter area (FSC-A) vs. FolateRSense fluorescence (APC). The percent of cells with internalized fluorescent folate and the mean fluorescence per cell type are shown.