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. 2015 Mar 27;10(3):e0122353. doi: 10.1371/journal.pone.0122353

Fig 3. Eye marker expression in PUbB2 P61 x HWE mosquitoes before and after CRISPR/Cas9 mediated ECFP knockout.

Fig 3

Eyes were viewed under a fluorescent stereo microscope (Leica M205) equipped with DsRed (A, C, E) or ECFP (B, D, F) specific filter sets. (A, B) Eyes of a PUbB2 P61 x HWE female. (C, D) Eyes of a (PUbB2 P61 x HWE) P41 female (G1) originating from an embryo which had been injected with in vitro transcribed RNAs encoding Cas9 and two ECFP targeting sgRNAs, sg13 and sg35. (E, F) Eyes of a HWE female. (G) Eyes of the HWE female under bright field.