Table 1. C4 mutant phenotype screen on N. benthamiana.
| C4 mutant 1 | Phenotype 2 | C4 mutant | Phenotype |
|---|---|---|---|
| Acidic amino acid mutations | Serine/Threonine mutants | ||
| C4E41A | C4-like | C4S12A | C4-like |
| C4E56A | C4-like | C4S18A or T | C4-like 4 |
| C4E69A | C4-like | C4S24A | C4-like |
| C4E73A | C4-like | C4S35A | C4-like |
| C4S48A | C4-like | ||
| Basic amino acid mutations | C4S49A | PVX-like | |
| C4K13A | C4-like 3 | C4A49T | C4-like |
| C4R40A | C4-like | C4S52A | C4-like |
| C4R54A | C4-like | C4S66A | C4-like |
| C4R65A | C4-like | C4T38A | C4-like |
| C4R84A | C4-like | C4T47A | C4-like |
| C4R85A | C4-like | C4T51A | C4-like |
| C4T55A | C4-like 5 | ||
| Double mutations | |||
| C4R40AE41A | C4-like | N-myristoylation mutation | |
| C4S52AR54A | C4-like | C4G2A | PVX-like |
1 Mutant designation, point mutants: C4(Original amino acid)(residue position)(New amino acid).
2 Phenotype was scored as C4-like or PVX-like at 14 days post inoculation.
3 Milder C4-like, less contorted stem and less discoloration along leaf veins
4 Very mild phenotype, leaf surface is smooth, no stem curling or petiole rolling, only mild leaf curling on older leaves.
5 Symptoms C4-like, but onset was delayed 2 to 3 days.