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Turbot were vaccinated with or without (control) pCN247, pCN523, or pCN3 and challenged with megalocytivirus. At 24 h post-challenge, immune gene expression in spleen was determined by quantitative real time RT-PCR. For convenience of comparison, for each gene the mRNA level of the control fish was set as 1. Data are presented as means±SE (N = 3). N, the number of times the experiment was performed. **P < 0.01; *P < 0.05.
