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. 2015 Mar 27;10(3):e0121439. doi: 10.1371/journal.pone.0121439

Fig 2. Luciferase reporter assay for the human ITGB6 gene promoter.

Fig 2

(A&B) The ITGB6 promoter-luciferase constructs are schematized. All numbers presented are relative to the TSS of the human ITGB6 gene. Luciferase reporter constructs containing various lengths of the ITGB6 promoter, or the promoter-less plasmid pGL2-basic, were co-transfected with pRL-TK into TCA8113 cells and 293T cells. Luciferase activity was measured at 48 hr after transfection. The luciferase value of the pGL2-basic was set to a value of one. Relative promoter activities are presented on the right (mean ± SEM, at least three independent experiments) and are analyzed by one-way ANOVA, where * p < 0.05, ** p < 0.01, and ** p < 0.001. (C) Nucleotide sequence of the main promoter region of the ITGB6 gene, depicting predicted binding sites for the transcriptional factors (underlined).