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. 2015 Mar 27;10(3):e0121439. doi: 10.1371/journal.pone.0121439

Fig 3. Involvement of TBP in the transcriptional regulation of ITGB6.

Fig 3

(A) Luciferase activity expressed by the TATA box directed mutant pGL2-B6-M-TA and wild type construct pGL2-B6(−150/+21) after being transfected into TCA8113 and 293T cells 48 hr. * p < 0.05 vs. WT. (B) ChIP assay was performed using anti-TBP antibody or IgG as a control in TCA8113 cells. Input and immunoprecipitated DNAs were then amplified by PCR using primer pairs covering the TATA box from −150 to +21. (C) TCA8113 cells transfected with siRNA against TBP, and the TBP protein expression determined by immunoblot analysis. (D) TCA8113 cells were co-transfected with pGL2-B6(−150/+21) and TBP siRNA or non-targeting control (NC) siRNA, and with pRL-RK. Relative luciferase activity was detected by dual luciferase reporter assay system. * p < 0.05 vs. NC siRNA. (E) SAS cells were transfected with TBP siRNA or NC siRNA for 48 hr. The mRNA expression levels of TBP and ITGB6 were determined by RT-qPCR. * p < 0.05 vs. NC siRNA.