Strains |
|
|
E. coli
|
|
|
S17–1(λpir) |
Tpr Smr, recA ths hsdRM
+, λpir phage lysogen RP4::Mu::Km Tn7
|
[23, 25] |
DH5α |
F-, φ 80dlacZ ΔM15, Δ(lacZYA-argF) U169, deoR, recA1, endA1, hsdR17 (rK
-, mK
+), phoA, supE44, λ
-, thi-1, gyrA96, relA1; Host of plasmid pRK2013 |
Our lab |
HB101 |
supE44, △ (mcrC-mrr), recA13, ara-14, proA2, lacY1, galK2, rpsL20, xyl-5, mtl-1, leuB6, thi-1; host of plasmid pBBR1MCS-5 |
Our lab |
Pseudoalteromonas sp. |
|
|
Rf-1 |
Wild type; spontaneously resistant to rifampicin |
This study |
B3 |
mutant with increased LAAO activity; Rifr, Kmr
|
This study |
B21 |
mutant with increased LAAO activity; Rifr, Kmr
|
This study |
B22 |
mutant with increased LAAO activity; Rifr, Kmr
|
This study |
B9 |
mutant with reduced LAAO activity; Rifr, Kmr
|
This study |
B10 |
mutant with reduced LAAO activity; Rifr, Kmr
|
This study |
B11 |
mutant with reduced LAAO activity; Rifr, Kmr
|
This study |
B17 |
mutant with reduced LAAO activity; Rifr, Kmr
|
This study |
B20 |
mutant with reduced LAAO activity; Rifr, Kmr
|
This study |
B1 |
LAAO-deficient mutant ; Rifr, Kmr
|
This study |
B6 |
LAAO-deficient mutant; Rifr, Kmr
|
This study |
B12 |
LAAO-deficient mutant; Rifr, Kmr
|
This study |
B19 |
LAAO-deficient mutant; Rifr, Kmr
|
This study |
Plasmids |
|
|
pLOF/Km |
Ori R6K, mob RP4, Apr, mini-Tn10 Kmr; delivery vector |
[24] |
pBBR1MCS-5 |
Rep, LacZ, Gmr; delivery vector for complementation |
[28] |
pRK2013 |
Derivative of IncP-1 plasmid RK2, Tra
+, ColE1, oriT, Kmr; assistant plasmid for complementation |
[29] |