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. 2015 Mar 27;10(3):e0122741. doi: 10.1371/journal.pone.0122741

Table 3. Bacterial strains and plasmids used in this study.

Strain or plasmid Description and/or relevant genotype Reference or source
Strains
E. coli
S17–1(λpir) Tpr Smr, recA ths hsdRM +, λpir phage lysogen RP4::Mu::Km Tn7 [23, 25]
DH5α F-, φ 80dlacZ ΔM15, Δ(lacZYA-argF) U169, deoR, recA1, endA1, hsdR17 (rK -, mK +), phoA, supE44, λ -, thi-1, gyrA96, relA1; Host of plasmid pRK2013 Our lab
HB101 supE44, △ (mcrC-mrr), recA13, ara-14, proA2, lacY1, galK2, rpsL20, xyl-5, mtl-1, leuB6, thi-1; host of plasmid pBBR1MCS-5 Our lab
Pseudoalteromonas sp.
Rf-1 Wild type; spontaneously resistant to rifampicin This study
B3 mutant with increased LAAO activity; Rifr, Kmr This study
B21 mutant with increased LAAO activity; Rifr, Kmr This study
B22 mutant with increased LAAO activity; Rifr, Kmr This study
B9 mutant with reduced LAAO activity; Rifr, Kmr This study
B10 mutant with reduced LAAO activity; Rifr, Kmr This study
B11 mutant with reduced LAAO activity; Rifr, Kmr This study
B17 mutant with reduced LAAO activity; Rifr, Kmr This study
B20 mutant with reduced LAAO activity; Rifr, Kmr This study
B1 LAAO-deficient mutant ; Rifr, Kmr This study
B6 LAAO-deficient mutant; Rifr, Kmr This study
B12 LAAO-deficient mutant; Rifr, Kmr This study
B19 LAAO-deficient mutant; Rifr, Kmr This study
Plasmids
pLOF/Km Ori R6K, mob RP4, Apr, mini-Tn10 Kmr; delivery vector [24]
pBBR1MCS-5 Rep, LacZ, Gmr; delivery vector for complementation [28]
pRK2013 Derivative of IncP-1 plasmid RK2, Tra +, ColE1, oriT, Kmr; assistant plasmid for complementation [29]