Table 6. Primers for the amplification of full length genes of sdmt, kari and nhad.

Primers	Oligonucleotide sequences	Restriction sites b
SdmtF a	5’-CCAAGCTTGGATGGATAAACCTTACTCTC-3’	Hind III
SdmtR	5’-CGGAATTCCGTTACTGCCGTTTAAAAATC-3’	EcoR I
KariF	5’- GGGGTACCCCATGGCGAACTATTTCAATT-3’	Kpn I
KariR	5’-CCAAGCTTGGTTATATTATTTTCTTCATC-3’	Hind III
NhaDF	5’-GGGGTACCCCATGAAAAACTCGCTATATGT-3’	Kpn I
NhaDR	5’-GCTCTAGAGCTCAAACACAGTGAATAGCTC-3’	Xba I

^a “F” refers to forward primer and “R” to reverse one;

^b Restriction sites are underlines.