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. 2015 Mar 27;10(3):e0122530. doi: 10.1371/journal.pone.0122530

Fig 3. The analytical sensitivity of the PML-RARa 3-plex RT-qPCR assay.

Fig 3

(A) Amplification plot for PML-RARa of serial plasmid dilutions with analytical sensitivity of 10 copies per reaction. (B) Amplification plot for ABL of serial plasmid dilutions with analytical sensitivity of 10 copies per reaction. (C) Amplification plot for no template control (NTC). (D) Amplification plot for 1μg RNA from PML-RARa positive NB4 cell line diluted in control RNA sample with analytical sensitivity of 10-4 (1 in 104) dilution. Dilutions of PML-RARa and ABL ranged from 106 to 100 copies, and dilutions of NB4 cell RNA ranged from 10-1 to 10-5.