Table 2. Primers and probes for 3-plex RT-qPCR of PML-RARa and ABL.
| Primer/probe a | Sequence (5′-3′) | GenBank Accession No. | Position |
|---|---|---|---|
| RT-PCR for PML-RARa | |||
| PML primer 1 | AGAGGATGAAGTGCTACGCCT | M73778 | 1081–1101 |
| RARA primer 2 | TTCCGGGTCACCTTGTTGAT | X06538 | 620–639 |
| RT-PCR for ABL | |||
| ABL primer 3 | GGGCTGTCCTCGTCCTCCA | X16416 | 193–211 |
| ABL primer 4 | CACCCTCCCTTCGTATCTCA | X16416 | 641–660 |
| 3-plex RT-qPCR for PML-RARa | |||
| PML primer 5 | CCCCAGGAAGGTCATCAAGA | M73778 | 1532–1551 |
| PML primer 6 | GACCTCAGCTCTTGCATCACC | M73778 | 1233–1253 |
| RARA primer 7 | GGCTTGTAGATGCGGGGTAG | X06538 | 486–467 |
| RARA probe (r) | FAM-TAGTGCCCAGCCCTCC-MGB | X06538 | 438–453 |
| 3-plex RT-qPCR for ABL | |||
| ABL primer 8 | ACCCAGTGAAAATGACCCCA | X16416 | 318–327 |
| ABL primer 9 | GATGTAGTTGCTTGGGACCCA | X16416 | 475–495 |
| ABL probe (r) | VIC-TGGGCTTCACACCATTC-MGB | X16416 | 439–455 |
aProbe (r) = Reverse TaqMan MGB probe; PML primer 1 for bcr1, bcr2 and bcr3; PML primer 5 for bcr1 and bcr2; PML primer 6 for bcr3