Table 3. Comparative results for 3-plex RT-qPCR and other methods from 319 patients with leukemia for transcripts of PML-RARa.
| Translocation/rearrangement | Patients | Karyotype | Conventional nested RT-PCR | 3-plex RT-qPCR |
|---|---|---|---|---|
| t(15;17)(q22;q12)/PML-RARA | 61 | 61 | 61 a | 61 |
| t(8;21)(q22;q22)/RUNX1-RUNX1T1 | 39 | 39 | 39 | 0 |
| inv(16)(p13.1q22) or t(16;16)(p13.1;q22)/ CBFB-MYH11 | 32 | 32 | 32 | 0 |
| t(9;11)(p22;q23)/MLLT3-MLL | 4 | 4 | 4 | 0 |
| t(6;9)(p23;q34)/DEK-NUP214 | 1 | 1 | 1 | 0 |
| t(9;22)(p34;q11.2)/ BCR-ABL1 | 61 | 61 | 61 | 0 |
| t(v;11q23)/MLL rearranged | 12 | 12 | 12 | 0 |
| t(12;21)(p13;q22)/TEL-AML1 | 14 | 14 | 14 | 0 |
| t(1;19)(q23;p13.3)/TCF3-PBX1 | 16 | 16 | 16 | 0 |
| No translocation/rearrangement | 79 | 79 | 0 | 0 |
| Total | 319 | 319 | 240 | 61 |
aThere were 33(54.1%) cases of PML-RARa bcr1, 3(4.9%) cases of PML-RARa bcr2 and 25 (41.0%) cases of PML-RARa bcr3.