Table 1. Effects of pH and Temperature on the Percentage of Escherichia coli O157:H7 Growth Inhibition after 24 hours ^a.

Incubation Temperature, ZnO NPs Concentration, µg.mL-1	Percentage of Escherichia coli O157:H7 Growth Inhibition at Different pH
	4	5	6	7	8	9	10
25ºC
1.5	100	100	100	100	100	100	100
0.75	63 ± 2.68	61 ± 0.7	35 ± 0.7	27 ± 0.42	33 ± 0.7	35 ± 1.02	35 ± 0.63
0.375	48 ± 0.84	45 ± 0.71	27 ± 0.01	20 ± 0.21	25 ± 0.42	25 ± 0.42	25 ± 0.21
37 ºC
1.5	100	100	100	100	100	100	100
0.75	79 ± 0.7	77 ± 0.98	52 ± 0.56	32 ± 0.91	41 ± 0.07	46 ± 0.56	49 ± 0.49
0.375	62 ± 2.21	59 ± 0.01	34 ± 0.55	25 ± 0.28	27 ± 0.14	29 ± 0.01	29 ± 0.91
42 ºC
1.5	100	100	100	100	100	100	100
0.75	87 ± 0.28	84 ± 1.97	69 ± 0.77	40 ± 0.49	52 ± 0.07	57 ± 0.35	57 ± 0.14
0.375	78 ± 0.7	75 ± 0.49	51 ± 2.47	33 ± 0.01	38 ± 0.28	40 ± 0.35	45 ± 0.42

^a Abbreviation: ZnO NP, zinc oxide nanoparticle.