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. 2015 Mar 18;16:7. doi: 10.1186/s12867-015-0037-5

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© Prieto et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Nop58 interacts with Nop17 through its C-terminal portion. (A) Schematics summarizing the results of two-hybrid assay with deletion mutants of Nop58 and Nop17. Nop58(324–512) mutant interacts with Nop17. (B) Co-immunoprecipitation of RNA with ProtA-Nop58 in the absence or presence of different amounts of Nop17. Incubation of total extract with IgG-sepharose beads was performed for 2 h at 4°C in the presence or absence of purified GST-Nop17. Co-immunoprecipitated U3 snoRNA was detected by northern blot. 5S rRNA was used as an internal control. (C) Quantitation of the U3 bands corrected by 5S bands after northern hybridization.