Figure 1.
HRM real-time qPCR analysis for the identification of 12S rRNA gene of canine filarioids. Normalized HRM curves of positive samples with Acanthocheilonema reconditum (blue) (Tm = 74.39 ± 0.03°C), Dirofilaria immitis (green) (Tm = 74.92 ± 0.04°C) and D. repens (red) (Tm = 75.54 ± 0.05°C).