Table 1.

Comparative detection of Dirofilaria immitis by different diagnostic assays

Diagnostic technique	Number of positive dogs	% of all dogs positive for Dirofilaria immitis by PCR or serology
HRM real-time PCR with sequencing and serology	22	100%
HRM-PCR with sequencing	17	77%
Serology	16	73%
KMT	13	59%
MCT	12	54%

Dogs positive by HRM real-time PCR and DNA sequencing or by specific serology were considered as truly positive. The table compares how many of these truly positive dogs were also positive by the Knott’s modified test (KMT) and the microcapillary test (MCT).