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. Author manuscript; available in PMC: 2016 Mar 28.
Published in final edited form as: Neuroscience. 2014 Sep 28;281:229–240. doi: 10.1016/j.neuroscience.2014.09.038

Figure 1. Effective knockdown of PAR-1 with PAR-1 shRNA lentivirus in brain sections and transfected neuronal cells.

Figure 1

(A) Lentivirus was injected into rat striatum. After one week the animals were sacrificed and brain sections were stained to determine the effective knockdown of PAR-1 mediated by lentivirus. Sections were stained with rabbit polyclonal antibody against PAR-1 (Alexa 594 goat anti-rabbit secondary antibody.) Pictomicrograph shows cells infected with lentivirus expressing a mis-sense RNA (misRNA) PAR-1 colocalize with PAR-1 protein indicated with arrows, demonstrating no knock down. Cells infected with shRNA PAR-1 lentivirus show no colocalization with PAR-1 receptor antibody, indicated with arrow heads. (B) Cell lysate taken from infected cells was used to determine the protein levels of PAR-1 with western blot analysis. Decreased expression of PAR-1 was observed in cells infected with PAR-1 shRNA when compared to control cells and PAR-1 misRNA infected cells. (C) Densitometry analysis shows a significant reduction in PAR-1 expression upon infection with PAR-1 shRNA lentivirus *** p<0.001.