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. Author manuscript; available in PMC: 2016 Jul 1.
Published in final edited form as: Insect Biochem Mol Biol. 2014 Sep 28;62:194–210. doi: 10.1016/j.ibmb.2014.09.007

Figure 6. Phylogeny of human, mouse and insect MGAT and DGAT proteins.

Figure 6

Alignments were produced with Clustal Omega and the phylogeny was inferred using the Neighbor-Joining method (Saitou and Nei, 1987). The bootstrap consensus tree inferred from 500 replicates is taken to represent the evolutionary history of the taxa analyzed (Felsenstein, 1985). Branches corresponding to partitions reproduced in less than 50% bootstrap replicates were collapsed. The evolutionary distances were computed using the JTT matrix-based method (Jones et al., 1992) and are in the units of the number of amino acid substitutions per site. The analysis involved 31 amino acid sequences. All positions containing gaps and missing data were eliminated. There were a total of 284 positions in the final dataset. Evolutionary analyses were conducted in MEGA5 (Tamura et al., 2011).

The sequence identifiers and abbreviations used for DGAT1 are: HumanDGAT1: Homo sapiens, NP_036211.2; MmDGAT1: Mus musculus, NP_034176.1; DpDGAT1: Danus plexipus; EHJ74688.1; HmDGAT1: Heliconius Melpomene, HMEL011652; MsexDGAT1: Manduca sexta; KF800701; BmDGAT1: Bombyx mori; XP_004927020.1; TcDG1: Tribolium castaneum; XP_975142.1; DmDGAT1: Drosophila melanogaster, NP_609813.1; AgDGAT1: Anopheles gambiae, XP_317656.3; AeaDG1: Aedes aegypti, XP_001658299.1; BtDGAT1: Bombus terrestris, XP_003400037.1; AmDGAT1: Apis mellifera XP_624754.2

Sequence identifiers for DGAT2 proteins: HumanDGAT2: Homo sapiens DGAT2 Isoform 1, Q96PD7; MmDGAT2: Mus musculus DGAT2, NP_080660.1.

Sequence identifiers for MGAT proteins: MsexMGAT352: Manduca sexta Predicted MGAT2 (352aa) KF800700; MsexMGAT346: Manduca sexta Predicted MGAT2 (346aa) KF800699; BmMGAT: Bombyx mori XP_004922894.1; DpMGAT: Danaus plexippus EHJ64327.1; TcMGAT: Tribolium castaneum, EEZ98240.1; NvMGAT: Nasonia vitripennis XP_001602288.1; HsMGAT: Harpegnathos saltator, EFN85928.1; HumanMGAT1: Homo sapiens MGAT1 NP_477513.2; MmMGAT1: Mus musculus MGAT1, NP_080989.2; MmMGAT2: Mus musculus MGAT2, Q80W94; HumanMGAT2: Homo sapiens MGAT2, NP_079374.2; HumanMGAT3: Homo sapiens MGAT3, NP_835470; DmCG1941: Drosophila melanogaster DGAT; DmCG1942: Drosophila melanogaster DGAT; DmCG1946: NP_610319; AgMGAT: Anopheles gambiae EDO63767.1; AeaMGAT: Aedes aegypti AAEL008878-PA.