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. 2015 Mar 24;15:69. doi: 10.1186/s12866-015-0402-z

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© Hansen et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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AHLs produced by the A. salmonicida ΔlitR mutant at different temperatures and cell densities. Supernatants were harvested at different time points during growth at 6, 12 and 16°C. The AHL concentrations were measured by HPLC-MS/MS and are shown with respect to time (hours) and cell density (OD₆₀₀). Symbols indicate the different AHLs; 3-oxo-C6-HSL (●), C6-HSL (□), 3-oxo-C8-HSL (▲), 3-OH-C10-HSL (○), 3-oxo-C10-HSL (■), C4-HSL (◇), 3-oxo-C4-HSL (◆) and C8-HSL (∆). Each value represents the mean of triplicates from three biological replicates. The error bars represent the standard deviations. The dotted lines in the top panels display the growth curves of the ΔlitR mutant at the different temperatures.