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. 2015 Mar 25;15:172. doi: 10.1186/s12885-015-1155-7

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© Yeh et al.; licensee BioMed Central. 2015

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Interrelationships among Ras, RbAp46 and RECK in clinical bladder cancer tissues. (A) The proteins extracted from the tissue of four clinical bladder cancer patients were evaluated by Western blotting to detect expression levels of Ras, RECK and RbAp46 using specific antibodies as indicated. β-actin was used as the internal control. N: normal tissue of bladder, and T: bladder tumor in patients. T24 cells were used as a positive control for the expression of mutant Ras and RECK. The intensity of bands for Ras (B), RECK (C) and RbAp46 (D) was quantified by a densitometer. Quantified intensity of bands was plotting using a Spearman correlation (E-G).