Table 2.
Primers used in this study
| Gene | Forward (5′-3′) | Reverse (5′-3′) | Function |
|---|---|---|---|
| sseA | GGGCTAAGGTGAGTCAACA | TGAAGAATACTCTCTGTCTCTCTG | Virulence (SPI-2 associated) |
| sseB | CATCTTATGGGGAAGTCAAAACC | GATAAGTCATCCTGGCTCCC | Virulence (SPI-2 associated) |
| ssah | TGATTTCCCAGGTACATGCGATG | GCCAACAATAATGCCAGACATACC | Virulence (SPI-2 associated) |
| sseG | GCCGATCCCAGTGTGTTATT | GCATTTGGGCTAACAGGTTTC | Virulence (SPI-2 associated) |
| ychP | GCGACCTTAGAACAGCGAAT | CCAAAGTACTGCTCCAGACTAAC | Invasion |
| cigR | CCCACATCAGAAGGGCAATATC | GCCATTACCATTTCCCGGATT | Inner membrane protein |
| safC | ATGGTAGCGCCATTCCTTTC | CCGCCAAACCAGTGAGATAAA | Outer membrane protein |
| ompF | CGTTGGCGCGAAATATGATG | GTTTGCCATTTCCACGGTATC | Outer membrane protein F |
| ybeF | TCCCGATCCGCTGTTTATTC | GCTTATCATAGCTGCCCGTAA | DNA transcription activator |
| FliH | TCGTCTGTCTCAGGTGGAA | CTTGCCACGGCAATTCATTAG | DNA transcription activator |
| sdiA | GCCCAGCGTTTCGGATTA | GTAAACGAGGAGCAGCGTAAA | DNA transcription, quorum sensing |
| trpA | CAGCCATTGTCAAGATTATCG | CTGAGACAAAGGACCTGAG | Tryptophan biosynthesis |
| argC | CAGTTTCTGTGAAGTGAGTTT | CAGATGGACGGCGATTTC | Arginine biosynthesis |
| dppA | ATCAAAGCCGTTTATCAG | TTAATATCGTCGTTGTAGC | ABC transporter system |
| artP | GTCTTTCAGCAATATAATCTTTGG | TCTTTTGTCAGACCCAGTA | ABC transporter system |
| livJ | CAACGGCGGCAAAGTATA | CGTACTGCTGCTTATCGT | ABC transporter system |
| 16 s rRNA | CAGAAGAAGCACCGGCTAAC | AATGCAGTTCCCAGGTTGAG | Endogenous control |