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. Author manuscript; available in PMC: 2016 Mar 1.
Published in final edited form as: Biochem J. 2015 Mar 1;466(2):273–281. doi: 10.1042/BJ20141159

Figure 4. Steady-state kinetic analysis of PRODH2.

Figure 4

(A) Rate versus substrate concentration plot for Hyp and L-proline. Reaction conditions: 50 mM HEPES pH 7.5, 200 nM PRODH2; 4 mM o-AB; 200 μM CoQ1, 25 °C. (B) Rate versus substrate concentration plot for the ubiquinone analogues menadione (▼), duroquinone (○), and CoQ1 (●). The same assay conditions were used holding the Hyp concentration constant at 600 mM.