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Constructs tested for PRODH2
| Construct | Solubility (%) | FAD loading (%) | kcat (s−1) |
|---|---|---|---|
| 1–536 | <1 | <1 | – |
| 77–536 | <1 | <1 | – |
| 94–536 | <1 | <1 | – |
| 128–536 | <10 | <1 | – |
| 147–536 | <50 | <1 | – |
| 157–536 | <50 | <1 | – |
| 165–536 | <50 | <1 | – |
| 173–536 | <50 | <1 | – |
| 1–515 | <1 | <1 | – |
| 147–515 | >90 | >96 | 0.22 ± 0.02 |
| 157–515 | >90 | >100 | 0.19 ± 0.01 |
| 165–515 | >90 | ~80 | – |
