Skip to main content
. Author manuscript; available in PMC: 2016 Mar 1.
Published in final edited form as: Biochem J. 2015 Mar 1;466(2):273–281. doi: 10.1042/BJ20141159

Table 2.

Kinetic parameters for ubiquinone analogues*

Analogue Km(μM) kcat(s−1) kcat/Km(M−1·s−1) Ki(μM)
Duroquinone 143 ± 18 0.27 ± 0.01 1900 ± 140
Menadione 25 ± 3 0.28 ± 0.01 11000 ± 770 306 ± 34
CoQ1 124 ± 19 0.19 ± 0.01 1500 ± 130
*

Reaction conditions: 50 mM HEPES pH 7.5, 4 mM o-AB, 200 nM PRODH2 157–515, 0–400 μM ubiquinone analogue, 600 mM Hyp.