Figure 2. Antioxidants reduce LD accumulation in mutant retinas.

A) a–c, Nile Red stains of 1-day-old sicily, Marf and Aats-met mutant retinas reveal LD accumulation. d–f, Animals fed with AD4 at 40µg/ml (AD4^high in quantification) reduces the number of LD. Notice the partial restoration of rhabdomere integrity in Marf mutants. g–i, Suppressing ROS by overexpression of a copy of hSOD1 (eyeless[ey]-Gal4, UAS-Flp) also reduces LD accumulations. B) Quantification of the data presented in A and animals with mutant clones raised on food supplemented with 20µg/ml (AD4^low) of AD4 the show a reduction of LD accumulation. C) Linear regressions show a negative correlation between the numbers of LD accumulated and the amount of supplemented AD4. D) Expression of hSOD1 with da-Gal4 in sicily mutants significantly suppresses the loss of aconitase activity and hence ROS E) Nile Red staining of wild type (Canton-S) flies show no LD accumulation whereas staining of SODⁿ¹ mutant escapers reveal high levels of LD accumulation. F) Quantification of SODⁿ¹ LD accumulation. Data are represented as mean ± SEM. Significance was calculated compared to controls using t-tests (*P<0.05, **P<0.005, ***P<0.0005).