| Time | Temperature | Purpose |
|---|---|---|
| 15 minutes | 50°C | Reverse Transcription |
| 2 minutes | 95°C | Denaturation |
| Cycle 18–20 times: | ||
| 15 seconds | 95°C | Denaturation |
| 4 minutes | 60°C | Annealing and elongation |
| Indefinitely | 4°C | End |
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