| Time | Temperature | Purpose |
|---|---|---|
| 2 minutes | 50°C | Thermal mixing |
| 30 minutes | 70°C | Thermal mixing |
| 10 minutes | 25°C | Thermal mixing |
| 2 minutes | 50°C | Uracil-N-glycosylase activation |
| 10 minutes | 95°C | Hotstart for Taq polymerase |
| Cycle 35 times | ||
| 15 seconds | 95°C | Denaturation |
| 1 minute | 60°C | Annealing and elongation |
| Ramp | 60°C to 95°C | Melting curve |
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