Table 3.
Anticytotoxicity of iCNSL and tCNSL as measured by the effect on the cell division rate of A. cepa roots (cm; mean ± standard deviation, and % cell division inhibition) at different concentrations and treatments with copper sulphate (1.2 μg·mL−1).
| Treatments (% inhibition) |
Controls/vehicle | iCNSL | tCNSL | ||||||
|---|---|---|---|---|---|---|---|---|---|
| NC | PC | TWEEN | Concentrations | Concentrations | |||||
| 17.37 µg·mL−1 | 34.75 µg·mL−1 | 69.50 µg·mL−1 | 17.37 µg·mL−1 | 34.75 µg·mL−1 | 69.50 µg·mL−1 | ||||
| Pretreatment (% inhibition) |
NT | NT | NT | 29.28 ± 6.37a∗∗∗;b∗∗
(44.54%) |
23.78 ± 0.68a∗∗∗
(54.96%) |
28.14 ± 1.33a∗∗∗;b∗∗
(46.70%) |
8.92 ± 0.75a∗∗∗
(83.10%) |
9.68 ± 1.46a∗∗∗
(81.66%) |
13.54 ± 3.44a∗∗∗
(74.35%) |
| Cotreatment (% inhibition) |
NT | NT | NT | 24.48 ± 3.89a∗∗∗;b∗
(53.63%) |
23.16 ± 1.91a∗∗∗
(56.13%) |
27.28 ± 2.16a∗∗∗;b∗∗
(48.33%) |
8.38 ± 0.84a∗∗∗
(84.12%) |
9.12 ± 1.46a∗∗∗
(82.72%) |
14.84 ± 2.76a∗∗∗
(71.89%) |
| Posttreatment (% inhibition) |
NT | NT | NT | 25.64 ± 1.86a∗∗∗
(51.43%) |
20.64 ± 2.85a∗∗∗
(60.90%) |
28.52 ± 1.58a∗∗∗;b∗∗∗
(45.98%) |
13.16 ± 1.23a∗∗∗
(75.07%) |
21.72 ± 2.08a∗∗∗
(58.86%) |
32.00 ± 1.30a∗∗∗;b∗∗∗
(39.39%) |
NC (dechlorinated water). PC (copper sulphate solution). TWEEN (solvent). NT (not tested). 1.000 cells evaluated per bulb, totalling 5,000 cells per group. aSignificant compared with the negative control; bsignificant compared with the positive control. * P < 0.05, ** P < 0.01, and *** P < 0.001 ANOVA. Tukey's test for multiple comparisons between groups.