Table 5.
Groups with concentration | Chromosome bridges | Chromosomal aberration/5000 cells | Total frequency (%) | |||
---|---|---|---|---|---|---|
Vagrant chromosomes | Laggard chromosomes | Fragments | Total frequency | |||
iCNSL pretreatment | ||||||
17,37 µg·mL−1 | 0.40 ± 0.89b∗ | 1.60 ± 0.50b∗∗∗ | 4.00 ± 4.84 | 2.00 ± 1.22 | 8.00 ± 3.46b∗∗∗ | 0.80 ± 0.34b∗∗∗ |
34,75 µg·mL−1 | 0.60 ± 0.89b∗ | 1.20 ± 1.09b∗∗∗ | 2.60 ± 2.96b∗ | 2.60 ± 1.94 | 7.00 ± 4.06b∗∗∗ | 0.70 ± 0.40b∗∗∗ |
tCNSL pretreatment | ||||||
17,37 µg·mL−1 | 1.20 ± 1.00 | 1.00 ± 0.00b∗∗∗ | 8.00 ± 4.41 | 1.20 ± 0.83b∗ | 11.40 ± 7.33b∗∗∗ | 1.14 ± 0.73b∗∗∗ |
34,75 µg·mL−1 | 0.75 ± 0.50 | 1.20 ± 0.44b∗∗∗ | 8.20 ± 3.11 | 2.40 ± 1.51 | 12.80 ± 3.76b∗∗∗ | 1.28 ± 0.37b∗∗∗ |
69,5 µg·mL−1 | 0.80 ± 0.40 | 1.60 ± 0.44b∗∗∗ | 8.00 ± 4.00 | 2.60 ± 1.81 | 13.00 ± 5.61b∗∗∗ | 1.30 ± 0.56b∗∗∗ |
iCNSL cotreatment | ||||||
17,37 µg·mL−1 | 0.40 ± 0.89b∗ | 1.00 ± 0.00b∗∗∗ | 3.40 ± 1.81b∗ | 1.60 ± 1.14 | 6.40 ± 2.51b∗∗∗ | 0.64 ± 0.25b∗∗∗ |
34,75 µg·mL−1 | 0.20 ± 0.44b∗ | 1.20 ± 0.44b∗∗∗ | 5.40 ± 2.60 | 1.00 ± 0.70b∗∗ | 7.80 ± 2.77b∗∗∗ | 0.78 ± 0.27b∗∗∗ |
tCNSL cotreatment | ||||||
17,37 µg·mL−1 | 0.60 ± 0.54b∗ | 1.60 ± 1.14b∗∗∗ | 5.20 ± 1.78 | 2.00 ± 1.00 | 9.40 ± 2.51b∗∗∗ | 0.94 ± 0.25b∗∗∗ |
34,75 µg·mL−1 | 0.60 ± 0.54b∗ | 1.20 ± 1.64b∗∗∗ | 4.60 ± 2.19 | 2.40 ± 1.14 | 8.80 ± 0.30b∗∗∗ | 8.80 ± 3.03b∗∗∗ |
69,50 µg·mL−1 | 1.60 ± 1.94 | 3.40 ± 1.67b∗∗∗ | 8.80 ± 5.35 | 3.40 ± 2.79 | 17.20 ± 5.76b∗∗∗ | 1.72 ± 0.57b∗∗∗ |
iCNSL posttreatment | ||||||
17,37 µg·mL−1 | 0.20 ± 0.44b∗∗ | 1.20 ± 0.83b∗∗∗ | 1.60 ± 1.57b∗∗ | 1.60 ± 1.14 | 4.60 ± 1.81b∗∗∗ | 0.46 ± 0.18b∗∗∗ |
34,75 µg·mL−1 | 0.20 ± 0,44b∗ | 2.00 ± 1.00b∗∗∗ | 4.00 ± 4.35 | 2.40 ± 0.89 | 8.60 ± 4.27b∗∗∗ | 0.86 ± 0.42b∗∗∗ |
tCNSL posttreatment | ||||||
17,37 µg·mL−1 | 1.20 ± 0.44 | 1.00 ± 1.22b∗∗∗ | 6.00 ± 4.84 | 1.00 ± 1.22b∗ | 9.20 ± 7.46b∗∗∗ | 0.92 ± 0.74b∗∗∗ |
34,75 µg·mL−1 | 0.80 ± 0.44 | 2.60 ± 1.51b∗∗∗ | 7.40 ± 2.52 | 2.60 ± 2.60 | 13.00 ± 6.51b∗∗∗ | 1.30 ± 0.65b∗∗∗ |
69,50 µg·mL−1 | 1.20 ± 0.44 | 3.80 ± 1.78b∗∗ | 7.00 ± 4.12 | 2.80 ± 0.83 | 14.80 ± 4.08b∗∗ | 1.48 ± 0.40b∗∗ |
NC (dechlorinated water). PC (copper sulphate solution). TWEEN (solvent). NT (not tested). 5.000 cells evaluated per bulb, totalling 5,000 per group. aSignificant compared with the negative control; bsignificant compared with the positive control. * P < 0.05, ** P < 0.01, and *** P < 0.001 ANOVA. Tukey's test for multiple comparisons between groups.