Figure 2.
Huaier blocked ADR-induced mitochondrial dysfunction in vitro. (a) Mitochondrial morphology imaged by transmission electron microscopy. (b) Representative images of podocytes stained with dichlorodihydrofluorescein diacetate (DCFDA). (c) Quantitation of 2′,7′-dichlorofluorescein (DCF) fluorescence by flow cytometry. (d) Quantitation of MitoSOX fluorescence by flow cytometry. Podocytes were pretreated with Huaier (0.2 mg/mL) for 1 h followed by incubation with ADR (200 nM) for further 24 h. Data are expressed as the means ± SD (n = 6). * P < 0.05 versus control. ** P < 0.05 versus ADR group.