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Primer sequences for real-time PCR.
| Genes | Forward (5′-3′) | Reverse (5′-3′) |
|---|---|---|
| Shh | CGGAGCGAGGAAGGGAAAG | TTGGGGATAAACTGCTTGTA |
| Gli1 | GGCTGGACCAGCTACATCAAC | TGGTACCGGTGTGGGACAA |
| Smo | ATCTCCACAGGAGAGACTGGTTCGG | AAAGTGGGCCTTGGGAACATG |
| DNMT1 | AACCAACACCCAAACAGAAACT | CTCCATCTTCGTCCTCGTCAG |
| β-actin | CGTGCTGCTGACCGAGG | GAAGGTCTCAAACATGATCTGGGT |
