Figure 1. Oligomers of PKD2L1 in mouse tissues and human cell lines.

(A) Detection of PKD2L1 in mouse kidney, testis, and brain tissues by WB. Samples were prepared with SDS loading buffer under the non-reducing and reducing (supplemented with 5% 2-ME or 0.5 M DTT) conditions. PKD2L1 was detected with an antibody from Abnova. Na/K ATPase was used as a loading control. Detection of over-expressed human PKD2L1 in HEK (B) and HeLa cells (C). pcDNA3.1(+) containing human PKD2L1 or empty vector (Ctrl) was transfected into HEK and HeLa cells. Cell lysates were collected 48 hr after transfection. Samples for SDS-PAGE were prepared under non-reducing or reducing condition. (D) Detection of over-expressed human PKD2L1 in HEK cells under non-reducing and reducing conditions. Samples were prepared with or without treatment by 10 mM NEM. (E) WB analysis after BN-PAGE of Flag-tagged PKD2L1 over-expressed in HeLa cells. Samples were prepared with or without 2.5% SDS, or with 2.5% SDS plus 0.1 M DTT. An anti-Flag antibody was used for WB detection. Putative PKD2L1 monomer, dimer and trimer are indicated.