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. 2015 Mar 30;5:9476. doi: 10.1038/srep09476

Figure 2. RT-PCR analysis of UBB and UBC mRNA in clinical NSCLC samples and the shRNA targeting ubiquitin silencing.

Figure 2

RT-PCR was used to detect the expression level of UBB (A) and UBC (B) mRNA in 24 paired lung tumor and normal tissues. RT-PCR analyzed the silencing efficiency of (C) four shRNA targeting UBB gene (shRNA-UBB) and (D) four shRNA targeting UBC gene (shRNA-UBC). Arrows indicate the amplification product of tandem repeat sequence of ubiquitin coding genes. (E) Western blot was used to detect the expression of ubiquitin protein level after transfection of indicated vectors. * P < 0.05, ** P < 0.01.