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. 2015 Mar 30;5:9476. doi: 10.1038/srep09476

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H1299 cells were transfected with indicated shRNAs and then sham irradiated or irradiated with 6 Gy X-ray. (A) Immunofluorescence of γH2AX foci (red) in H1299 cells for an additional 0, 0.5, 1, 2, 4 or 12 h. Nuclei were stained with DAPI (blue). Images were acquired using a fluorescent microscope. (B) Kinetics of γH2AX foci loss in different groups of cells after 6 Gy X-ray irradiation. The mean number of γH2AX foci per cell (foci/cell) was counted. * P < 0.05, compared with shRNA-NC group. (C) Western blot was performed to detect the γH2AX foci induced by X-ray irradiation after transfection with indicated shRNA vectors.