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. 2015 Mar 30;5:9476. doi: 10.1038/srep09476

Figure 7. Knock-down of ubiquitin affected AKT activation and inhibited NF-κB translocate induced by X-ray irradiation.

Figure 7

H1299 cells were transfected with indicated shRNA for 24 h prior to sham irradiation or 6 Gy X-ray irradiation. (A) Western blot showing the nuclear and cytoplasmic distribution of NF-κB with sham irradiation or 6 Gy X-ray irradiation in H1299 cells. (B) Immunofluorescence analysis of NF-κB distribution in nucleus and cytoplasm of H1299 cells. (C)The expression levels of NF-κB, AKT, p-AKT, p-IκBα, Cyclin D1 and the internal control β-actin were measured by Western blot. The samples were derived from the same experiment and were run under the same experimental conditions. The uncropped blots details are provided in Supplementary Information.