Table 1.

Recommended indications for use of laser microdissection/mass spectrometry-based proteomics for amyloid typing in kidney biopsies

Absence of tissue available for immunofluorescence microscopy

Negative immunofluorescence staining for kappa and lambda light chains AND negative immunohistochemical staining for serum amyloid A protein

Equal immunofluorescence staining for both kappa and lambda light chains.

Strong immunofluorescence staining for immunoglobulin heavy chains, with or without light chain staining.

Positive immunofluorescence staining for kappa and/or lambda light chains AND positive immunohistochemical staining for serum amyloid A protein.

Equivocal Congo red staining.

Table reproduced (with permission) from Said et al. [7].