Figure 4. HFD-fed TCRδ^−/− mice have fewer proinflammatory macrophages in eAT after 5 weeks of HFD.

(A) Flow cytometric analysis of F4/80⁺CD11b⁺ macrophages and F4/80⁺CD11c⁺ M1 macrophages in eAT of 5-week-old WT and TCRδ^−/− mice at baseline before starting on HFD. Data are represented as number of cells/fat pads and number of cells/fat pads/gram fat; n = pool of eAT from 5 mice, 2–3 pools/group. (B) F4/80 mRNA expression in eAT of TCRδ^−/− and TCRδ^+/+ mice after 5 weeks of ND or HFD (n = 6–9 mice in each group) and in eAT of HFD-fed C57BL/6J mice treated with IgG isotype control antibody or anti-TCRγδ antibody (n = 5 mice in each group). *P < 0.05 by Student’s t-test, as determined by qPCR analysis. (C–E) Flow cytometric analysis of SVF cells in eAT of TCRδ^−/− and TCRδ^+/+ mice after 5 weeks of ND or HFD. (C) Total F4/80⁺ macrophages, represented as percentage of cells within macrophage gate based on scatter pattern, (D) TNF-α⁺ macrophages, and (E) F4/80⁺CD11c⁺CD206^– M1, F4/80⁺CD11c^–CD206⁺ M2, and F4/80⁺CD11c^–CD206^– DN macrophages; n = 6–9 mice in each group. Data from a representative experiment of 2 independent experiments. Data are represented as number of cells/fat pads and number of cells/fat pads/gram of fat and as a percentage of macrophages. Values are mean ± sem. *P < 0.05, **P < 0.01, and ***P < 0.001 by one-way ANOVA with Tukey’s post hoc test. Numbers inside boxes refer to percent of cells, and *P < 0.05 comparing HFD-fed groups of mice.