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. 2014 Nov 13;97(1):121–134. doi: 10.1189/jlb.3A0414-211RR

Figure 5. Quantification of macrophages in eAT of TCRδ−/− mice after 10 weeks of HFD.

Figure 5.

(A) mRNA expression of F4/80 and CD11c in eAT of TCRδ−/− and TCRδ+/+ mice after 10 weeks of ND or HFD, as determined by qPCR analysis; n = 5–6 mice in each group. (B–D) Flow cytometric analysis of SVF cells in eAT of TCRδ−/− and WT mice after 10 weeks of ND or HFD. (B) Total F4/80+ macrophages, represented as percentage of cells within macrophage gate based on scatter pattern, (C) TNF-α+ macrophages, and (D) F4/80+CD11c+CD206– M1, F4/80+CD11c–CD206+ M2, and F4/80+CD11c–CD206– DN macrophages; n = 4–5 mice in each group. Data from a representative experiment of 2 independent experiments. Data are represented as number of cells/fat pads and number of cells/fat pads/gram of fat. Values are mean ± sem. *P < 0.05, **P < 0.01, and ***P < 0.001 by one-way ANOVA with Tukey’s post hoc test.