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. 2014 Oct 28;97(1):171–180. doi: 10.1189/jlb.4A0214-087R

Figure 4. GC-rich binding site at −613 to -603 bp of Elk-3 promoter is important for LPS-induced down-regulation of Elk-3 promoter.

Figure 4.

RAW 264.7 cells were transiently transfected with (1) WT promoter construct Elk-3 (–775/+387); (2) Elk-3 (–775/+387), with GC-rich region –19 to +112 bp deleted; (3) Elk-3 (–775/+387), with mutation of GC-rich region–613 to –603 bp; or (4) both GC-rich regions disrupted. Along with the promoter constructs, an expression plasmid for β-galactosidase was transfected to correct for transfection efficiency. The cells were allowed to recover overnight and were then exposed to LPS (100 ng/ml) or vehicle for 6 h. Luciferase activity for each group is presented as mean ± sem in the presence (+) or absence (–) of LPS, n = 9/group (from 3 independent experiments), comparing LPS-exposed cells with its control in each group by unpaired t-test. P values are provided above the bars or no significance. Comparisons between groups are made by one-way ANOVA (P < 0.0001). Significant comparisons; * versus WT Elk-3 (−775/+387) + LPS.