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. 2014 Oct 23;97(1):191–200. doi: 10.1189/jlb.5A1113-626RR

Figure 5. DA inhibits ERK and JNK signaling in monocytic MDSCs.

Figure 5.

(A) Immunoblot (upper) of iNOS expression by purified CD115+ MDSCs from tumor-bearing mice, stimulated with or without 50 ng/ml IFN-γ for 24 h in the presence or absence of 5 or 10 µM DA. (Lower) The intensity of the bands was quantitated. *P < 0.05, and **P < 0.01; (B) Immunoblot of p-p38, total p38, p-ERK, total ERK, p-JNK, and total JNK by purified CD115+ MDSCs stimulated with 50 ng/ml IFN-γ for 1 or 3 h in the presence or absence of 50 µM DA. β-Actin was immunoblotted as a loading control in A and B. (C) NO production by purified CD115+ MDSCs pretreated with 10 µM U0126 or 25 µM SP600125 for 30 min, followed by 24 h stimulation with 50 ng/ml IFN-γ. Data representing 4 independent experiments, yielding similar results, are shown. ***P < 0.001. Error bars indicate sem.