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. 2014 Oct 23;97(1):191–200. doi: 10.1189/jlb.5A1113-626RR

Figure 6. MDSCs in cancer patients express D1-like DA receptors, and their agonist inhibits NO production.

Figure 6.

(A) D1 and D5 expression by Lin‒ CD33+HLA-DR‒ MDSCs, sorted from peripheral blood of lung cancer patients. Shaded histograms indicate the staining by use of isotype control antibodies. (B) Flow cytometric measurement of intracellular NO levels by Lin‒CD33+HLA-DR‒ MDSCs from lung cancer patients pretreated with vehicle or 10 µM SKF38393 and then stimulated with 50 ng/ml IFN-γ for 24 h. Shaded histograms indicate the fluorescence of cells without IFN-γ stimulation. (C) The mean fluorescence intensity (MFI) of intracellular NO level in human MDSCs stimulated with IFN-γ alone or with IFN-γ and SKF38393. The data shown summarize 3 independent experiments. **P < 0.01. Error bars indicate sem.