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. Author manuscript; available in PMC: 2015 Mar 30.
Published in final edited form as: Obesity (Silver Spring). 2010 Jan 28;18(5):872–878. doi: 10.1038/oby.2009.512

Figure 4.

Figure 4

Effect of fasting on gene expression of developmental and adipogenic transcription factors genes in C57BL/6 mice and ob/ob Mice. Expression of the six developmental transcription factors Shox2, En1, Tbx15, Hoxc9, Hoxc8 Hoxa5 (a,b) and four adipogenic transcription factors Cebpb, Cebpd, Cebpa, and Pparg2 (c,d), were measured in six different fat depots before and after fasting in both lean C57BL/6 mice (a,c) and ob/ob mice (b,d). Differences in gene expression before and after fasting in the same depot were analyzed by Student's t-test. Note that Hoxc8 in white fat pads shown decreased trend by fasting in both C57BL/6 mice and ob/ob mice. Expression of each gene in the different fat depots is indicated as open circles (Flk), filled circles (Bck), open squares (Epd), filled squares (Prr), open triangles (Msn), open diamonds (BAT). Differences in gene expression before and after fasting in the same depot from five independent samples each from a different mouse (n = 5) were analyzed by Student's t-test (*significance at P < 0.05).