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. 2014 Dec 18;15(1):1134. doi: 10.1186/1471-2164-15-1134

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© Wong et al.; licensee BioMed Central. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Summary of validation of hyperosmotic effects on intestinal transcription factors discovered from transcriptome by real-time PCR. The transcription factors were examined, and 19/57 were reproducible. Among the candidate genes, 5/19 were found to be specific towards hyperosmotic challenge (red) while others were related to general transfer stress (blue). Small graphs are representative real time PCR results of each category: stress-related, TSC22D3 and SGK1; salinity-related, CEBPD; insignificant change, SBNO2. Statistical significant groups are indicated by asterisks in the bar graphs (Two-way ANOVA, Bonferroni with time-matched comparison.*p < 0.05).