Table 1.
Illumina sequencing and mapping statistics
| Sample | Treatment | Number of reads | Number of mapped | Number of unique read | Number of multiple | Number of unmapped | % mapped | % unique | % concordantly mapped pair/mapped pair |
|---|---|---|---|---|---|---|---|---|---|
| medaka_DT1 | FW | 45,187,196 | 38,886,015 | 36,800,226 | 2,085,798 | 6,301,181 | 86.06 | 81.44 | 96.04 |
| medaka_DT2 | FW | 60,510,650 | 51,531,207 | 48,089,685 | 3,441,522 | 8,979,443 | 85.16 | 79.47 | 96.32 |
| medaka_DT3 | FW | 49,132,444 | 42,149,176 | 39,787,090 | 2,362,086 | 6,983,268 | 85.79 | 80.98 | 96.01 |
| medaka_DT4 | FW | 53,098,918 | 45,320,354 | 42,439,720 | 2,880,634 | 7,778,564 | 85.35 | 79.93 | 96.12 |
| medaka_DT5 | FW | 42,600,708 | 36,530,248 | 34,556,499 | 1,973,749 | 6,070,460 | 85.75 | 81.12 | 96.46 |
| medaka_DT6 | SW1h | 42,628,084 | 36,663,691 | 34,708,187 | 1,955,504 | 5,964,393 | 86.01 | 81.42 | 95.96 |
| medaka_DT7 | SW1h | 56,932,366 | 48,827,425 | 46,194,052 | 2,633,373 | 8,104,941 | 85.76 | 81.14 | 96.90 |
| medaka_DT8 | SW1h | 42,093,406 | 36,151,083 | 34,098,610 | 2,052,473 | 5,942,323 | 85.88 | 81.01 | 96.47 |
| medaka_DT9 | SW1h | 52,158,762 | 44,893,371 | 42,420,952 | 2,472,419 | 7,265,391 | 86.07 | 81.33 | 96.05 |
| medaka_DT10 | SW1h | 36,707,006 | 31,548,390 | 29,929,884 | 1,618,506 | 5,158,616 | 85.95 | 81.54 | 96.06 |
| medaka_DT11 | SW3h | 41,952,606 | 36,052,499 | 34,168,827 | 1,883,672 | 5,900,107 | 85.94 | 81.45 | 95.05 |
| medaka_DT12 | SW3h | 38,901,344 | 33,331,036 | 31,561,823 | 1,769,213 | 5,570,308 | 85.68 | 81.13 | 96.60 |
| medaka_DT13 | SW3h | 48,830,644 | 42,173,994 | 39,875,418 | 2,298,576 | 6,656,650 | 86.37 | 81.66 | 94.54 |
| medaka_DT14 | SW3h | 43,038,240 | 37,145,021 | 35,132,181 | 2,053,293 | 6,445,624 | 85.80 | 81.28 | 96.36 |
| medaka_DT15 | SW3h | 45,401,548 | 38,955,924 | 36,902,631 | 2,053,293 | 6,445,624 | 85.80 | 81.28 | 96.36 |
| medaka_DT16 | SW1d | 41,811,642 | 35,924,612 | 34,060,638 | 1,863,974 | 5,887,030 | 85.92 | 81.46 | 96.24 |
| medaka_DT17 | SW1d | 27,329,496 | 23,684,730 | 22,440,772 | 1,243,958 | 3,644,766 | 86.66 | 82.11 | 95.70 |
| medaka_DT18 | SW1d | 26,278,316 | 22,719,337 | 21,476,111 | 1,243,226 | 3,558,979 | 86.46 | 81.73 | 95.82 |
| medaka_DT19 | SW1d | 20,440,212 | 20,139,877 | 19,080,869 | 1,059,008 | 3,300,335 | 85.64 | 81.09 | 96.01 |
| medaka_DT20 | SW7d | 23,440212 | 20,139,877 | 19,080,869 | 1,059,008 | 3,300,335 | 85.92 | 81.40 | 96.56 |
| medaka_DT21 | SW7d | 20,399,688 | 17,475,541 | 16,546,834 | 928,707 | 2,924,147 | 85.67 | 81.11 | 96.54 |
| medaka_DT22 | SW7h | 17,289,434 | 14,563,145 | 13,793,496 | 769,649 | 2,726,289 | 84.23 | 79.78 | 96.92 |
| medaka_DT23 | SW7h | 23,527, 904 | 19,851,220 | 18,617,357 | 1,233,863 | 3,676,684 | 84.37 | 79.13 | 97.13 |
| medaka_DT24 | SW7h | 27,563,352 | 23,083,585 | 21,713,537 | 1,370,048 | 4,479,767 | 83.75 | 78.78 | 97.48 |
| medaka_DT25 | SW7h | 21,133,038 | 18,209,017 | 17,192,056 | 1,016,961 | 2,924,021 | 86.16 | 81.35 | 96.18 |
DT: digestive tract; FW: freshwater; SW: 50% seawater.