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. 2014 Dec 17;15(1):1130. doi: 10.1186/1471-2164-15-1130

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© Cao et al.; licensee BioMed Central. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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qRT-PCR validation of chilling-responsive conserved and novel miRNAs in ‘LA1777’ (a) and ‘Hezuo908’ (b) tomato. The reference gene was 5S rRNA. All reactions were repeated three times. Normalized miRNA expression amount at 1 h without chilling treatment was arbitrarily set to 1. Differences between the NT and CT libraries were tested with a T test. *indicates a significant difference between the two libraries (<0.05).