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. 2015 Mar 21;5:12. doi: 10.1186/s13578-015-0002-5

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© Liu et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Infiltration of macrophages in DSS-treated WT and GHSR ^−/− colons. Colonic infiltrating macrophages were examined by immunohistochemical staining of F4/80. More F4/80⁺ cells were observed in the submucosal region of inflamed WT colon (A) than GHSR^−/− (B). In addition, the numbers F4/80⁺ cells in the mucosal and submucosal layer were counted, and the final results were showed as numbers of F4/80⁺ cells in per mm² mucosa (C). Each bar represents the mean ± SEM; *p < 0.05, **p < 0.01, and ***p < 0.005.